043-0131/15-7000 - Acquisition of Microscope for Department of Chemistry & Nano-Science

The instrument should perform standard TIRF- and wide field fluorescence microscopy with single molecule resolution. Perform simultaneous multicolour and FRET imaging under wide field and TIRF illumination. The emission light from donor and acceptor shall be split and imaged simultaneous on either two different EM-CCD cameras or on a single camera using dual camera adaptor. Software for analysis of data should be included. The instrument should perform photoswitching of photoactivatable chromophores with at least 2 lasers. The instrument should perform single molecule imaging with signal to noise ratio of >5 under ideal conditions. Higher S/N is desired. The instrument should perform single molecule localization s.d. of ±40 nm or less (fitting centroid position of PSFs)

13/07/2015 12:00:00

38510000-3  Microscopes